Rabbit anti-Ebola Virus GP BIOTIN-Conjugated Polyclonal Antibody

• Catalog number
  494-0301-015-bio
• Price
  Please ask
• Size
  100 μg

• Lot number
  1503001
• Description
  Affinity purified rabbit polyclonal antibody reactive to EBOV GP. The antibody detects GP in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region (EBOV rGPdTM) in Western blot and ELISA. Purified rabbit polyclonal antibody reactive to EBOV GP was conjugated with biotin and purified from unconjugated biotin by diafiltration. Antibody was conjugated with biotin at a molar ratio of 20:1 (Biotin: antibody)
• Virus category
  Filoviruses
• Type
  polyclonal antibody
• Viral target
  Ebola Virus (EBOV)
• Immunogen
  Peptide sequence to Ebola virus (EBOV) glycoprotein (GP).
• Raised in
  Rabbit
• Clonality
  polyclonal
• Applications
  Enzyme-Linked Immunosorbent Assay, Western Blot
• Storage
  2-3 weeks +4°C, -20°C long term
• Techfile
• Virus detection
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by virus detection they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by virus detection. Rabbit antibodies are very stable and can be stored for several days at room temperature. virus detection adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  Virus   Conjugated  
• Short name
  Rabbit anti- Virus GP -Conjugated Polyclonal Antibody
• Technique
  Polyclonal, Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Label
  Biotin
• Species
  Virus, Viruses
• Alternative name
  production species: rabbit Antibody toEbola Virus GP biotinilated-coupled polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies, rabbit-anti
• Disease
  Marburg or Ebola viruses ( EBOV ) need to be handled in BSL4 Biological safety level 4. However the monoclonal and polyclonal antibodies, ELISAs and recombinant proteins do not need any specific safety measure because no active virus is involved. The Zaire Ebola strain is called ZEBOV. The Sudan virus is called SEBOV.

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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