Heat Shock Protein 90 (Hsp90), human recombinant

• Catalog number
  4858-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Heat Shock Protein 90, HSP90
• Alternative_names
  Heat Shock Protein 90, HSP90
• Description
  A chaperone protein that assists protein folding
• Recombinant
  Yes
• Source
  Sf9 cells
• Purity by SDS PAGE
  ≥99%
• Assay
  SDS-PAGE
• Biological activity
  Not available at this time
• Molecular Weight
  83.1kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Concentration
  5 mg/ml
• Appearance
  Liquid
• Physical form description
  5mg/ml in 40 mM HEPES/KOH, pH 7.5, containing 400 mM potassium chloride and 5% glycerol
• Background Information
  Hsp90 is a molecular chaperone and is one of the most abundant proteins in unstressed cells. In mammalian cells, there are two genes encoding cytosolic Hsp90 homologues, with the human Hsp90α showing 85% sequence identity to Hsp90β. The recombinant Hsp90 produced from Sf9 cells represents the full length (a.a. 1-723) of Hsp90 subunit.
• Amino acid sequence
  PEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Heat   Shock   Protein   Hsp90  
• Gene symbol
  HSP90AA1
• Short name
  Heat Shock Protein 90 (Hsp90), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Heat Shock Protein 90 (Hsp90), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:5253
• Gene
  HSP90AA1
• Long gene name
  heat shock protein 90 alpha family class A member 1
• Synonyms gene
  • HSPC1
  • HSPCA
• Synonyms gene name
  • heat shock 90kD protein 1, alpha
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
• Synonyms
  • Hsp89
  • Hsp90
  • FLJ31884
  • HSP90N
• GenBank acession
  • M27024
• Locus
  14q32.31
• Discovery year
  1990-06-27
• Entrez gene record
  3320
• Pubmed identfication
  • 2527334
  • 16269234
• RefSeq identity
  • NM_005348
• Classification
  • Heat shock 90kDa proteins
• VEGA ID
  OTTHUMG00000171752

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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