Heat Shock Protein 70, human recombinant

  • Catalog number
    4859-25
  • Price
    Please ask
  • Size
    25 ug
  • Synonyms
    Human Heat Shock Protein 70, Heat Shock Protein 70
  • Alternative_names
    Human Heat Shock Protein 70, Heat Shock Protein 70
  • Description
    A chaperone protein that assists protein folding
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥98%
  • Assay
    SDS-PAGE
  • Purity
    ≥98%
  • Molecular Weight
    70.0 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized protein
  • Physical form description
    Lyophilized from 41 mM Tris, pH 8 containing 81 mM NaCl.
  • Reconstitution Instructions
    Reconstitute in H₂O (solubility > 40 mg/ml), aliquot and store at -80 °C
  • Background Information
    HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress. Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner. HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop, and p23. The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments. When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome. The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules. They also play a role in vesicle formation and protein trafficking.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    Heat   Shock   Protein  
  • Gene symbol
    HSPA13, HSPA9
  • Short name
    Heat Shock Protein 70, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    Heat Shock Protein 70, H. sapiens Rec.
  • Alternative technique
    rec
Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family A (Hsp70) member 13
  • Synonyms gene
  • Synonyms gene name
    • stress 70 protein chaperone, microsome-associated, 60kD
    • stress 70 protein chaperone, microsome-associated, 60kDa
    • heat shock protein 70kDa family, member 13
  • Locus
  • Discovery year
    1997-03-21
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Heat shock 70kDa proteins
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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