Heat Shock Protein 27, human recombinant

• Catalog number
  4853-1000
• Price
  Please ask
• Size
  1 mg

• Alternative_names
  Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Short name=HSP 27, Stress-responsive protein 27
• Description
  A chaperone protein that assists protein folding
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Biological activity
  Immunoreactivity is confirmed by reaction with monoclonal mouse antibodies against HSP-27.
• Molecular Weight
  22.7 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution containing 20 mM Hepes (pH 7.5), 1 mM DTT and 100 mM KCl.
• Background Information
  Recombinant Human Heat Shock Protein produced in E.Coli is a single, non-glycosylated polypeptide chain containing 205 amino acids and have a molecular weight of 22.7 kDa. The protein was purified by proprietary chromatographic techniques.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Heat   Shock   Protein  
• Gene symbol
  HSPE1P27, DNAJC27
• Short name
  Heat Shock Protein 27, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Heat Shock Protein 27, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:49346
• Gene
  HSPE1P27
• Long gene name
  heat shock protein family E (Hsp10) member 1 pseudogene 27
• Synonyms gene name
  • heat shock 10kDa protein 1 pseudogene 27
• Locus
  1p36.12
• Discovery year
  2013-10-18
• VEGA ID
  OTTHUMG00000002776

Gene info

• Identity
  HGNC:30290
• Gene
  DNAJC27
• Long gene name
  DnaJ heat shock protein family (Hsp40) member C27
• Synonyms gene
  • RBJ
• Synonyms gene name
  • rab and DnaJ domain containing
  • DnaJ (Hsp40) homolog, subfamily C, member 27
• Synonyms
  • RabJS
• Locus
  2p23.3
• Discovery year
  2007-07-31
• Entrez gene record
  51277
• Pubmed identfication
  • 14980719
• RefSeq identity
  • NM_016544
• Classification
  • DNAJ (HSP40) heat shock proteins
• VEGA ID
  OTTHUMG00000125524

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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