Mouse Anti-Human PINK1 Monoclonal IgG1, Clone S4-15
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Catalog numberSMC-450D-FITC
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PricePlease ask
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Size100 µg
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CloneS4-15
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ImmunogenFusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. 82% identical to rat and 81% identical to mouse. >30% identity with DMPK.
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Antibody s full descriptionMouse Anti-Human PINK1 Monoclonal IgG1 Antibody, Clone: S4-15: FITC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameParkinson disease (autosomal recessive) 6 Antibody, EC 2.7.11.1 Antibody, PARK6 Antibody, serine/threonine-protein kinase PINK1 mitochondrial Antibody, PTEN-induced putative kinase protein 1 Antibody, protein kinase BRPK Antibody, BRPK Antibody, FLJ27236 Antibody, PTEN induced putative kinase 1 Antibody, Phosphatase and Tensin Homolog Antibody
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Raised inMouse
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Antibody s targetPINK1
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Primary research fieldsNeuroscience, Cell Signaling, Post-translational Modifications, Phosphorylation, cardiovascular System, Heart
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~50kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-450 was sufficient for detection of PINK1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMitochondrion , Mitochondrion Outer Membrane , Cytoplasm
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextPINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports; PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction.
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Released date26-Sep-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_115785.1
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Gene number65018
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Protein numberQ9BXM7.1
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (SMC-450). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (SMC-450) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PINK1 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~50 kDa PINK1 protein using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (SMC-450). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (SMC-450) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa. Mouse Anti-PINK1 Antibody [S4-15] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-450) Mouse Anti-PINK1 Antibody [S4-15] used in Western Blot (WB) on Rat Brain (SMC-450)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolPINK1-AS, PINK1, PIRC67, PIRC66, PIRC71, PIRC75, PIRC68, PIRC62, PIRC72, PSMC1
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Short nameMouse Anti- PINK1 Monoclonal IgG1, Clone S4-15
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman phosphatase and tensin homolog induced putative kinase 1 monoclonal IgG1, clonality S4-15
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Alternative techniquemurine, antibodies
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Alternative to gene targetPTEN induced putative kinase 1, PINK1 and IDBG-92922 and ENSG00000158828 and 65018, transferase activity, nuclei, Pink1 and IDBG-199773 and ENSMUSG00000028756 and 68943, PINK1 and IDBG-645657 and ENSBTAG00000006084 and 510683
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Clone nameS4-15
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Gene info
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Identity
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Gene
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Long gene namePINK1 antisense RNA
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Synonyms gene
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Synonyms gene name
- PINK1 antisense RNA 1 (non-protein coding)
- PINK1 antisense RNA (non-protein coding)
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Synonyms
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GenBank acession
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Locus
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Discovery year2010-08-19
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Pubmed identfication
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePTEN induced kinase 1
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Synonyms gene
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Synonyms gene name
- Parkinson disease (autosomal recessive) 6
- PTEN induced putative kinase 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 67
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 66
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 71
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 75
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 68
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 62
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 72
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameproteasome 26S subunit, ATPase 1
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Synonyms gene name
- proteasome (prosome, macropain) 26S subunit, ATPase, 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-11-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Proteasome
- AAA ATPases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data