AKT1 Mouse monoclonal antibody
-
Catalog number
MB9025-50ul
-
Price
Please ask
-
Size
50ul
-
-
Alternative Name
RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα;
-
Immunogen
This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of AKT1.
-
Swiss Prot
P31749
-
Reactivity
Human, mouse
-
Applications
WB ICC IHC FC
-
Application_all
WB: 1:2000 IHC/ICC: 1:100-1:200 FC: 100-1:200
-
Product
Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
-
Purification Purity
Protein A affinity purified
-
Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
-
Specificity
This antibody detects endogenous levels of AKT1 and does not cross-react with related proteins.
-
Usage
Western blot (WB) analysis of AKT1 Mouse mAb at 1:2000 dilution Lane1:HepG2 whole cell lysate Lane2:MCF7 whole cell lysate Lane3:Hela whole cell lysate
-
BiowMW
~ 60 kDa
-
Note
For research use only, not for use in diagnostic procedure.
-
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
AKT1
-
Short name
AKT1 Mouse monoclonal antibody
-
Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
-
Host
Mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
-
Species
Mouse, Mouses
-
Alternative name
v-akt murine thymoma viral oncogene homolog 1 Mouse monoclonal (antibody to-)
-
Alternative technique
antibodies, murine
-
Alternative to gene target
v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
