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Alernative name
Rabbit Anti-Human NFkB p65 Polyclonal
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Target
NFkB p65
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Conjugate
FITC
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Research Area s
Cancer , Apoptosis , Neuroscience , Neurodegeneration , Alzheimer's Disease , Cell Signaling , Epigenetics and Nuclear Signaling , Nuclear Signaling Pathways , NFkB Pathway , Cardiovascular System , Atherosclerosis , Vascular Inflammation , Inflammatory Mediators , Cell Signaling , Metabolism
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
A synthesized peptide derived from human NF-kappaB p65
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Immunogen Species
Human
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Gene ID
5970
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Swiss Prot
Q04206
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Applications
WB , IHC , ICC/IF , IP
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Host Species
Rabbit
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Species Reactivity Full Name
Human , Mouse , Rat
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Storage Buffer
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Concentration
NA
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Specificity
Detects ~69kDa, endogenous levels of total NF-kappaB p65
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Storage Temperature
-20°C
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Shipping Temperature
Blue Ice or 4°C
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
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French translation
anticorps