NBR1, Autophagy substrate NBR1 (affinity purified)
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Catalog number
AS14 2805A
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Price
Please ask
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Size
50 µg
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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
UNA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS14 2805 | anti-NBR1 | Autophagy substrate NBR1, rabbit antibodies in serum format AS14 2769 | anti-ATG8, rabbit antibodiesAS14 2811 | anti-ATG8A | Autophagy-related protein 8a, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 500 - 1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent
75 | 100 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Brassicaceae family
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
specific extraction method and tissue type needs to be used as described in Minina et al. (2013). Dilution in western blot depends upon amount of NBR1 in the sample.
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References
to be added when available, antibody release in February 2015
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Scientific context
NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.
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Notes
None
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Protein number
Q9SB64
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TAIR number
AT4G24690
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Test
A high affinity purification column was use to purify NBR1, Autophagy substrate NBR1 ( ) by agisera by chromatographic size exclusion.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum.
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Gene target
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Gene symbol
NBR1
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Short name
NBR1, Autophagy substrate NBR1 ( )
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Technique
affinity, purified, purifications
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Alternative name
NBR1, Autophagy substrate NBR1 (protein enriched)
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Alternative technique
pure
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Gene info
Product images
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