MCP-1 (MCAF), human recombinant

• Catalog number
  4222-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  MCP1, SCYA2
• Alternative_names
  HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte chemotactic protein 1, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2
• Description
  Chemoattractant for monocytes and basophils
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Biological activity
  The activity is determined by its ability to chemoattract human monocytes at 4 - 15 ng/ml.
• Results
  4 - 15 ng/ml.
• Molecular Weight
  8.6 kDa
• Storage Temp
  -20°C
• Shipping
  Blue ice
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Sterile filtered and lyophilized with no additives
• Reconstitution Instructions
  Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Background Information
  Monocyte Chemoattractant Protein 1 (MCP-1), also known as CCL2, is thought to be produced by injured or infected tissues. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells. Recombinant mouse MCP-1 is a non-glycosylated protein, containing 76 amino acids and having a molecular mass of 8.6 kDa.
• Amino acid sequence
  QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  MCP-1   MCAF  
• Gene symbol
  CCL2, CAPG, CCL13, CCL8, SLC25A33, CCR2, CD46, CCL7, IGHVII-1-1, MIR1-1
• Short name
  MCP-1 (MCAF), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  MCP-1 (MCAF), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:10618
• Gene
  CCL2
• Long gene name
  C-C motif chemokine ligand 2
• Synonyms gene
  • SCYA2
• Synonyms gene name
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
  • chemokine (C-C motif) ligand 2
• Synonyms
  • MCP1
  • MCP-1
  • MCAF
  • SMC-CF
  • GDCF-2
  • HC11
  • MGC9434
• Synonyms name
  • monocyte chemotactic protein 1, homologous to mouse Sig-je
  • monocyte chemoattractant protein-1
  • monocyte chemotactic and activating factor
  • monocyte secretory protein JE
  • small inducible cytokine subfamily A (Cys-Cys), member 2
• GenBank acession
  • BC009716
• Locus
  17q12
• Discovery year
  1990-07-05
• Entrez gene record
  6347
• Pubmed identfication
  • 2004761
• RefSeq identity
  • NM_002982
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000132887

Gene info

• Identity
  HGNC:1474
• Gene
  CAPG
• Long gene name
  capping actin protein, gelsolin like
• Synonyms gene
  • AFCP
• Synonyms gene name
  • capping protein (actin filament), gelsolin-like
• Synonyms
  • MCP
• Synonyms name
  • macrophage capping protein
• GenBank acession
  • M94345
• Locus
  2p11.2
• Discovery year
  1993-12-14
• Entrez gene record
  822
• Pubmed identfication
  • 1322908
  • 12754261
• RefSeq identity
  • NM_001747
• Classification
  • Gelsolin/villins
• VEGA ID
  OTTHUMG00000130183

Gene info

• Identity
  HGNC:10611
• Gene
  CCL13
• Long gene name
  C-C motif chemokine ligand 13
• Synonyms gene
  • SCYA13
• Synonyms gene name
  • small inducible cytokine subfamily A (Cys-Cys), member 13
  • chemokine (C-C motif) ligand 13
• Synonyms
  • MCP-4
  • NCC-1
  • SCYL1
  • CKb10
  • MGC17134
• GenBank acession
  • AJ001634
• Locus
  17q12
• Discovery year
  1997-02-11
• Entrez gene record
  6357
• Pubmed identfication
  • 8661057
• RefSeq identity
  • NM_005408
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000132890

Gene info

• Identity
  HGNC:10635
• Gene
  CCL8
• Long gene name
  C-C motif chemokine ligand 8
• Synonyms gene
  • SCYA8
• Synonyms gene name
  • small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)
  • chemokine (C-C motif) ligand 8
• Synonyms
  • MCP-2
  • HC14
• GenBank acession
  • X99886
• Locus
  17q12
• Discovery year
  1996-11-14
• Entrez gene record
  6355
• Pubmed identfication
  • 9119400
• RefSeq identity
  • NM_005623
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000132883

Gene info

• Identity
  HGNC:29681
• Gene
  SLC25A33
• Long gene name
  solute carrier family 25 member 33
• Synonyms gene name
  • solute carrier family 25, member 33
  • solute carrier family 25 (pyrimidine nucleotide carrier), member 33
• Synonyms
  • MGC4399
  • BMSC-MCP
  • PNC1
• GenBank acession
  • AF495714
• Locus
  1p36.22
• Discovery year
  2006-09-21
• Entrez gene record
  84275
• Pubmed identfication
  • 14715278
  • 16949250
• RefSeq identity
  • NM_032315
• Classification
  • Solute carriers
• VEGA ID
  OTTHUMG00000001322

Gene info

• Identity
  HGNC:1603
• Gene
  CCR2
• Long gene name
  C-C motif chemokine receptor 2
• Synonyms gene
  • CMKBR2
• Synonyms gene name
  • chemokine (C-C motif) receptor 2
• Synonyms
  • CC-CKR-2
  • CKR2
  • MCP-1-R
  • CD192
  • FLJ78302
• Locus
  3p21.31
• Discovery year
  1995-05-30
• Entrez gene record
  729230
• Pubmed identfication
  • 8146186
• RefSeq identity
  • NM_000647
• Classification
  • C-C motif chemokine receptors
  • CD molecules
• VEGA ID
  OTTHUMG00000156466

Gene info

• Identity
  HGNC:6953
• Gene
  CD46
• Long gene name
  CD46 molecule
• Synonyms gene
  • MIC10
  • MCP
• Synonyms gene name
  • antigen identified by monoclonal antibody TRA-2-10
  • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)
  • CD46 antigen, complement regulatory protein
  • CD46 molecule, complement regulatory protein
• Synonyms
  • TRA2.10
  • MGC26544
  • TLX
• GenBank acession
  • BC030594
• Locus
  1q32.2
• Discovery year
  1988-08-31
• Entrez gene record
  4179
• Pubmed identfication
  • 7929741
• RefSeq identity
  • NM_172361
• Classification
  • Sushi domain containing
  • Complement system regulators and receptors
  • CD molecules
• VEGA ID
  OTTHUMG00000036397
• Locus Specific Databases
  • LRG_155

Gene info

• Identity
  HGNC:10634
• Gene
  CCL7
• Long gene name
  C-C motif chemokine ligand 7
• Synonyms gene
  • SCYA6
  • SCYA7
• Synonyms gene name
  • small inducible cytokine A7 (monocyte chemotactic protein 3)
  • chemokine (C-C motif) ligand 7
• Synonyms
  • MCP-3
  • NC28
  • FIC
  • MARC
  • MCP3
• Synonyms name
  • monocyte chemoattractant protein 3
  • monocyte chemotactic protein 3
• GenBank acession
  • AF043338
• Locus
  17q12
• Discovery year
  1993-11-04
• Entrez gene record
  6354
• Pubmed identfication
  • 8461011
• RefSeq identity
  • NM_006273
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000132889

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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