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Category
Primary Antibodies
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Long description
Mucins are the major glycoprotein component of mucous and their function is to protect epithelia from adverse conditions such as dehydration, infection, physical and chemical injury. They are large glycoproteins which vary in size and exist as either membrane bound or secreted molecules. The secreted molecules are subdivided into gel-forming and non gel-forming mucins. Mucin 5AC (MUC5AC) is a high molecular weight gel-forming glycoprotein, which is primarily expressed in the goblet cells of the respiratory epithelium, gastric epithelium and reproductive mucosa. The aberrant expression of MUC5AC has been reported in some cancers, including gastric, colon, endometrial adenocarcinomas and pancreatic cancer, suggesting a potential role for MUC5AC as a marker of these neoplasia. Increased levels of MUC5AC have also been reported in some pathological conditions such as asthma and cystic fibrosis.
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Antibody come from
45M1 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with M1 mucin purified from fluid taken from an ovarian mucinous cyst.
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Other description
Each vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.
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Clone
45M1
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Antigen antibody binding interaction
Mouse anti Mucin MUC5AC Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rat, Pig, Guinea pig, Hamster, Dog, Avian
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
The 45M1 antibody is directed against human mucin 5AC and has been reported to cross-react with avian, mouse, rat, hamster, guinea-pig , porcine, and canine mucin 5AC. The 45M1 antibody is reported to react specifically with an epitope located in the N-terminal region of the C-terminal cysteine-rich part of MUC5AC.
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Research interest
Extracellular matrix
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Application
ELISA, Flow cytometry, Immunocytochemistry, Immunohistochemistry (frozen), Immunohistology (paraffin), Immunoprecipitation, Western Blotting
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Antibody s suited for
The 45M1 antibody is suitable for the detection of mucin 5AC by ELISA, flow cytometry, western blotting and immunoprecipitation. The antibody is also suitable for the detection of MUC5AC by immunocytochemistry and immunohistochemistry on frozen tissues. Staining of paraffin embedded tissues with 45M1 is possible following heat mediated antigen retrieval of paraffin sections using citrate buffer. Optimal antibody dilutions for the different applications should be determined by titration.
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Storage
Store at 4oC, or in small aliquots at -20oC.
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Relevant references
1. Phillips JE, Case NR, Celly C, Chapman RW, Hey JA, Minnicozzi M (2006) An enzyme-linked immunosorbent assay (ELISA) for the determination of mucin levels in bronchoalveolar lavage fluid. J. Pharmacol. Toxicol. Methods 53:160-7. 2. Bara J, Gautier R, Mouradian P, Decaens C, Daher N (1991) Oncofetal mucin M1 epitope family: characterization and expression during colonic carcinogenesis. Int. J. Cancer 47:304-10. 3. Lidell ME, Bara J, Hansson GC (2008) Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin. FEBS J 275:481-9.
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Protein number
UniProt: P98088 (Human)
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
