Streptavidin, Conjugated, for ELISA

• Catalog number
  AS15 2860
• Price
  Please ask
• Size
  1 mg

• Available ordering format
  Inquire
• Immunogen
  to be determined
• Raised in
  N/A
• Clonality
  N/A
• Clone
  N/A
• Purification
  Affinity purified
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Store freeze-dried (lyophilized) powder at 2-8 °C. Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.Shelf life of this product is 1 year from date of receipt.
• Verified applications
  This conjugate is designed to be used in the detection of biotinylated primary antibodies. It is suitable for use in ELISA, WB, IHC.
• Connected products
  no related to other products
• Recommended dilutions for use
  The optimal working dilution should be determined by the investigator.Suggested starting dilution(s):1:500-1:2 000 for Immunohistochemistry1:50 000-1:5 000 for ELISA/Western blot
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  to be determined
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Buffer composition: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % Kathon CG (v/v) is included as preservative. Streptavidin is overexpressed in bacterial culture, purified and conjugated to horse radish peroxidase (HRP).
• References
  to be added when available, product released in March 2015.
• Scientific context
  Streptavidin is a protein that has a very high affinity for biotin (known as vitamin B7 or H). The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is mostly used in molecular biology and bionanotechnology since the complex of streptavidin-biotin is resistant to organic solvents, denaturants etc. and extremes of temperature and pH.
• Notes
  None
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

• Gene target
  Streptavidin   Conjugated   for  
• Short name
  Streptavidin, Conjugated, for ELISA
• Technique
  ELISA, antibody Conjugates, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
• Host
  antigen assay ELISA kit 1 Kit (96 Wells)
• Label
  streptavidin
• Alternative name
  biotinilated-binding streptavidin, coupled, to measure Enzyme-linked immunosorbent assay
• Alternative technique
  elisas

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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