Reusable Protein A/G conjugated Sepharose beads with ≥20 mg/ml binding capacity & high flow rate for antibody purification from multiple sources. Protein A/G Sepharose is prepared by covalently coupling recombinant Protein A/G (contains five Ig-binding regions of protein A and three Ig-binding regions of protein G, Cat. 6502) to 6% cross-linked sepharose beads. The coupling was optimized to give a high binding capacity for IgG. The capacity of IgG binding could be greater than 10 mg of human IgG per ml of wet gel.
•CONTENTS- Supplied as a 50% slurry in 20 % Ethanol/H₂O. ; >5mg Protein A/G per ml Sepharose beads. •FEATURES- Binding capacity greater than 10 mg/ml of wet gel; High flow rate; Low falling off of rProtein A/G; pH stability 2-10. •APPLICATIONS- Purification of monoclonal and polyclonal antibodies.