UbcH5b, human recombinant (His-tag)

• Catalog number
  4852-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  UbcH5b, Ubiquitin conjugating enzyme H5b, Ubiquitin conjugating enzyme
• Alternative_names
  UbcH5b, Ubiquitin conjugating enzyme H5b, Ubiquitin conjugating enzyme, Ubiquitin-conjugating enzyme E2 D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2
• Description
  An essential ubiquitin-conjugating enzyme
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Molecular Weight
  18.0 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Concentration
  1.0 mg/ml
• Appearance
  Liquid
• Physical form description
  Supplied at a concentration of 1.0 mg/ml in 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT.
• Background Information
  UbcH5b is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5b, together with UbcH5a and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53. Useful for in vitro ubiquitinylation reactions.The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support in vitro conjugation is 100 nM to 1 µM depending upon the conditions.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  UbcH5b   His-tag  
• Short name
  UbcH5b, recombinant (His-tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  UbcH5b, H. sapiens Rec. (histidine-detection labelled)
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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