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Description
Cy3 conjugated Annexin V for detection of apoptosis through fluorescence microscopy or by flow cytometry. The bright red color fluorescent reagent for detecting of the early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca²ᶧ-dependent affinity for PS and therefore serves as a probe for detecting apoptosis. The Annexin V-Cy3 conjugate contains the bright red fluorescent probe that can be used for detection of apoptosis by fluorescence microscopy with a rhodamine filter or by flow cytometry. Cy3 yields red fluorescence with a λmax emission of 570 nm.
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Product Highlights
•Detection method- flow cytometry (Ex/Em- 543/570 nm) using FL2 channel and fluorescence microscopy •Sample- Cell culture •Simple, easy to use and convenient
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Storage Temp
+4°C
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Shipping
gel pack
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Shelf Life
12 months
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Notes
For research use only. Not to be used for human or animal treatment or consumption.
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Additional description
This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.
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Properties
Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Annexin V- Reagent can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Biovision antibody is well suited for fluorescent microscopy.
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Conjugation
cy3 conjugation kit
