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Synonyms
Human Cellexp Human Recombinant Activin A
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Alternative_names
Inhba, Inhibin beta A, FSH releasing protein, FRP
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Description
A protein complex enhancing FSH biosynthesis and secretion
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Recombinant
Yes
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Source
Human cell expressed
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per 1 ug of protein
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Biological activity
ED₅₀ is typically 0.5 to 5 ng/mL. The specific activity was determined by the dose-dependent inhibition of proliferation of the MPC-11 cell line (mouse plasmocytoma cell line).
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Results
0.5 to 5 ng/ml
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Molecular Weight
25 kDa, homodimer, nonglycosylated.
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Storage Temp
-20°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from a PBS solution.
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Reconstitution Instructions
Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.
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Background Information
Activins are homodimers or heterodimers of the different subunit isoforms, part of the TGF-β super-family. Activin displays an extensive variety of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, hematopoiesis, and reproductive physiology. Activins takes part in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells that are identified to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells. BioVision’s Activin A is produced in a serum-free, chemically defined media.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants