Goat, F(ab)'2 fragment, FITC conjugated

• Catalog number
  AS10 1076
• Price
  Please ask
• Size
  0.5 mg

• Available ordering format
  Liquid
• Immunogen
  to be determined
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified IgG, F(ab)'2 fragment
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Store at 2-8°C. Shelf life of this product is one year from date of receipt.
• Verified applications
  Contact Gentaur support center
• Connected products
  other fluorescent dye conjugated secondary antibodies to human IgA
• Recommended dilutions for use
  1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  to be determined
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  Refer to PubMed
• Scientific context
  Goat anti-human IgA (alpha chain), FITC (fluorescein-5-isothiocyanate) conjugated, F(ab)'2 fragment of a secondary antibody which binds to human IgA in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
• Notes
  This antibody reacts with the alpha chains on human IgA based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus

• Gene target
  conjugated  
• Short name
  Goat, F(ab)'2 , FITC conjugated
• Technique
  FITC, Goat, antibody Conjugates, Fluorescein, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
• Label
  FITC
• Species
  Goats
• Alternative name
  caprine, F(Antibody)'2 fragment, fluorecein coupled
• Alternative technique
  caprine, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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