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Available ordering format
Lyophilized
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Immunogen
BSA-conjugated synthetic peptide derived from Arabidosis thaliana Lhcb5 protein UniProt: Q9XF89, TAIR: AT4G10340 sequence. This sequence is highly conserved in Lhcb5 proteins from monocots, dicots and conifers but only partial conserved in Physcomitrella patens and Chlamydomonas reinhardtii.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunohistochemistry (IHC)
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Connected products
AS01 009PRE Lhcb5 | CP26 chlorophyll a/b-binding protein of plant PSII, pre-immune serumAS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodiesAS01 011 Chlamydomonas | A set of anti-Lhc antibodies for ChlamydmonasAvailable antibodies against pigment-binding proteins- LHCrecommended secondary antibodyPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1 : 1000, detected with standard ECL (WB)
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Molecular weight expected аpparent
30 | 25-26 kDa for Arabidopsis thaliana
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Verified reactivity
Arabidopsis thaliana, Hordeum vulgare, Drosera capensis, Oryza sativa, Pisum sativum, Spinacia oleracea, Zea mays
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Possible reactivity
angiosperms (monocots and dicots), gymnosperms
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Protein is processed into mature form (Jansson 1999).
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References
Pavlovic et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008 Sun et al. (2014). Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes. Biochim Biophys Acta. 2014 Nov 22. pii: S0005-2728(14)00650-1. doi: 10.1016/j.bbabio.2014.11.005 Ido et al. (2014). Cross-Linking Evidence for Multiple Interactions of the PsbP and PsbQ Proteins in a Higher Plant Photosystem II Supercomplex. J Biol Chem. 2014 Jul 18;289(29):20150-7. doi: 0.1074/jbc.M114.574822. Epub 2014 Jun 9. Zhou et al. (2013) Mutation of the Light-Induced Yellow Leaf 1 Gene, Which Encodes a Geranylgeranyl Reductase, Affects Chlorophyll Biosynthesis and Light Sensitivity in Rice. PLoS ONE 8(9): e75299. doi:10.1371/journal.pone.0075299.
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Scientific context
Lhcb5 is one of the 3 minor highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.
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Notes
None
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Protein number
Q9XF89
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TAIR number
AT4G10340
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About
Plants RNA, DNA , cDNA and plant tissues are used to study plant genes.