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Also known as
HSPA5 / GRP78 / BIP
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Other names
78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; epididymis secretory sperm binding protein Li 89n; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
HSPA5
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Gene name synonims
GRP78; BIP
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Other gene names
HSPA5; HSPA5; BIP; MIF2; GRP78; HEL-S-89n; GRP78; GRP-78; BiP
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG
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Clone
N/A
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Host organism
Rabbit (Oryctolagus cuniculus)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes endogenous levels of total human BiP protein at 78kD. Species cross-reactivity: mouse.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Tissue culture supernatant
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Form Appearance
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
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Concentration
N/A
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Storage and shipping
For long term storage, keep the antibody at -20 degrees Celsius. Short term storage of the antibody can be done at +4 degrees Celsius. Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity.
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Tested applications
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Gene symbol
HSPA5, HSPA5-DT
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Short name
Monoclonal (IgG) HSPA5 / GRP78 / BIP Antibody
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Technique
Antibody, IgG, antibodies against human proteins, antibodies for, IgGs, Monoclonals or monoclonal antibodies
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Isotype
IgG, IgG
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Species
Human, Humans
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Alternative name
Anti-Rabbit Monoclonal (IgG) to Human HSPA5 / GRP78 / BIP
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Alternative technique
antibodies, immunoglobulins
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Alternative to gene target
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), BIP and GRP78 and HEL-S-89n and MIF2, HSPA5 and IDBG-85148 and ENSG00000044574 and 3309, misfolded protein binding, nuclei, Hspa5 and IDBG-163151 and ENSMUSG00000026864 and 14828
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