Our specialists from Gentaur/Genprice advise to follow carefully the instructions for use and store the COL12A1 Polyclonal Antibody, FITC Conjugated in refrigerator and according to the label upon arrival.
If you buy Antibodies supplied by Epigen they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Epigen Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Epigen FITC is highly sensitive to pH extremes.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
COL12A1 Polyclonal Antibody, FITC Conjugated
Polyclonal, Antibody, FITC
collagen, classification XII, a 1 polyclonal (Antibody to), fluorecein coupled