Stat3 (phospho-S727) polyclonal antibody
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Catalog number
AP0248-50ul
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Price
Please ask
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Size
50ul
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Alternative Name
Signal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRF
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Immunogen
Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Serine 727.
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Swiss Prot
P40763
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Reactivity
Human,Mouse,Rat
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Applications
WB
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Application_all
WB:1:500~1:1000
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificity
p-Stat3 (S727) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Ser727.
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Usage
Western blot (WB) analysis of p-Stat3 (S727) pAb at 1:2000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug)
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BiowMW
~ 88 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Short name
Stat3 (phospho-S727) polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit
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Alternative name
signal transducer and activator on transcription 3 (acute-phase response factor) (phosphorilated-S727) polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
signal transducer and activator of transcription 3 (acute-phase response factor), ADMIO and APRF and HIES, STAT3 and IDBG-50702 and ENSG00000168610 and 6774, protein dimerization activity, nuclei, Stat3 and IDBG-211637 and ENSMUSG00000004040 and 20848, STAT3 and IDBG-640197 and ENSBTAG00000021523 and 101910056,508541
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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