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ELISA s application
For quantitative measurement of human ADAM9 in cell culture supernatant, serum and plasma heparin .
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Gene tested
human ADAM9
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ELISA standard curve
Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.042 + 0.092 + 0.121 + 0.194 + 0.319 + 0.570 + 1.023 + 2.010
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ELISA kit detection limits
15.6pg/ml to 1000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural and recombinant human ADAM9
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);145;363;594;176;380;658 ELISA standard deviations 7.54;20.69;36.23;11.44;28.12;51.98 CV(%);5.2;5.7;6.1;6.5;7.4;7.9
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ELISA kit description
GENTAUR's human ADAM9 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for ADAM9 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, A29-D697) and your samples are measured with a biotin conjugated polyclonal from goat specific for ADAM9 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human ADAM9 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human ADAM9 antibodies;1 ST0000-10;Lyophilized recombinant human ADAM9 standard;10ng/tube×2 AR1107;Biotinylated anti- human ADAM9 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml human ADAM9 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin) to the ELISA plate. Keep in mind that each human ADAM9 standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
15-20
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More gene info
Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM('a disintegrin and metalloproteinase') family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.
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Antigen
NSO, A29-D697
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Disintegrin and metalloproteinase domain-containing protein 9
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Gene name
ADAM metallopeptidase domain 9
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Alternative
ADAM 9;ADAM metallopeptidase domain 9;Adam9;ADAM9_HUMAN;Cellular disintegrin-related protein;Cone rod dystrophy 9;CORD9;Disintegrin and metalloproteinase domain-containing protein 9;MCMP;MDC9;Meltrin-gamma;Metalloprotease/disintegrin/cysteine-rich protein 9;Mltng;Myeloma cell metalloproteinase
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Gen protein identity
Q13443
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Entrez nr
8754
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
ADAM9
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Short name
ADAM9 PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
H. sapiens ADAM metallopeptidase domain 9 PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
ADAM metallopeptidase domain 9, CORD9 and MCMP and MDC9 and Mltng, ADAM9 and IDBG-18369 and ENSG00000168615 and 8754, laminin binding, Extracellular, Adam9 and IDBG-143125 and ENSMUSG00000031555 and 11502, ADAM9 and IDBG-629406 and ENSBTAG00000017455 and 281600
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