• SIZE: 100 applications (5 µl per lane) • VOLUME: 500 µl • FORMULATION: 100 µg/ml in 10 mM Tris-HCl, pH 8.0, 10 mM EDTA, bromophenol blue and glycerol. • SOURCE: PCR products and double-stranded DNA digested with appropriate restriction enzymes then phenol extracted and equilibrated to 10 mM Tris-HCl , pH 8.0 with 1 mM EDTA. • STORAGE: Store at 25 °C for 6 months; -20 °C for 24 months.
BioVision’s BriteRuler™ 1 kb DNA Ladder is a ready-to-use formulation containing bromophenol blue as the tracking dye and is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The 1 kb and 3 kb bands have increased intensity to provide internal orientation. The mass of DNA in each band is provided (assuming a 0.5 µg/5 µl load) for approximate quantification in comparably intense samples of similar size. The DNA Ladder features 13 bands spanning the range from 250 bp to 10 kb. The recommended usage is 5 µl/Lane
Maximum time for storage
Ready to use DNA ladder for sizing linear double-stranded DNA fragments from 250 bp to 10 kb.