Glycine Oxidase H244K, Bacillus subtilis recombinant

• Catalog number
  7845-250
• Price
  Please ask
• Size
  250 ug

• Synonyms
  Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO
• Alternative_names
  Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO
• Description
  ≥90% Pure Active Bacillus subtilis recombinant Glycine Oxidase H244K which shows a higher substrate specificity ratio for glycine versus sarcosine
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  1200 mU/mg
• Biological activity
  1200 mU/mg
• Results
  1200 mU/mg
• Unit Definition
  One unit is defined as the amount of enzyme required to convert one micromole of glycine into glyoxylate and hydrogen peroxide at pH 8.5 at 37°C.
• Molecular Weight
  43.1 kDa (1-369 aa, NT His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  4.0 mg/ml
• Appearance
  Liquid
• Physical form description
  4.0 mg/ml in 20 mM potassium phosphate, 100 mM NaCl, pH 8.0 and 10% glycerol
• Background Information
  Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.
• Amino acid sequence
  1-369 aa
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Bacillus Anthracis and Cereus are the most known Bacilli. Anthrax has a very high toxin level and is used in Biologic warfare. However 1 is a harmless product.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Glycine   Oxidase   H244K   Bacillus   subtilis  
• Short name
  Glycine Oxidase H244K, Bacillus subtilis recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Glycine Oxidase H244K, Bacillus subtilis Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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