OVA Conjugated Fluorescein Isothiocyanate (FITC)

• Catalog number
  CPO843Ge21
• Price
  Please ask
• Size
  500ug

• Source
  Protein conjugation
• Predicted Molecular Mass KD
  389.4g/mol
• Accurate Molecular Mass KD
  NA
• Organism species
  Pan-species (General)
• Item Name
  Fluorescein Isothiocyanate
• Alternative Names
  FITC-Tag
• Research Area
  NA
• Buffer Formulation
  PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Traits
  Freeze-dried powder
• Purity
  > 95%
• Protein length
  NA
• Tag
  N-terminal His Tag
• Expression System
  Protein conjugation
• Application
  Immunogen; SDS-PAGE; WB.
• Manual link
• Properties
  This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Gene
  Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.

• Gene target
  OVA   Conjugated   Fluorescein   Isothiocyanate  
• Short name
  OVA Conjugated Fluorescein Isothiocyanate (FITC)
• Technique
  FITC, antibody Conjugates, Fluorescein
• Label
  FITC
• Species
  Pan-species (General)
• Alternative name
  OVA coupled Fluorescein Isothiocyanate (fluorecein)
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products