Isopeptidase T (long form), human recombinant

• Catalog number
  4862-25
• Price
  Please ask
• Size
  25 ug

• Synonyms
  USP5; Ubiquitin-specific protease 5
• Alternative_names
  USP5; Ubiquitin-specific protease 5
• Description
  A ubiquitin-specific processing protease
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Molecular Weight
  97.0 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Concentration
  0.4 mg/ml
• Appearance
  Liquid
• Physical form description
  Supplied at a concentration of 0.4 mg/ml in 50 mM Tris, 10 Mmn β-mercaptoethanol, pH 7.5.
• Background Information
  Isopeptidase T is a ubiquitin-specific processing protease (UBP) that specifically disassembles free K48-linked polyubiquitin chains into ubiquitin monomers. There are two human isopeptidase T isoforms; Isopeptidase T short form (isoT-S) and Isopeptidase T long form (isoT-L) which differ by an insertion of 23 amino acids in isoT-L. Isopeptidase T is the only UBP known to require zinc binding to be active.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Isopeptidase   long   form  
• Short name
  Isopeptidase T (long form), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Isopeptidase T (large form), H. sapiens Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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