This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Histone H2B polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Alternative names of antibody target:
Western blotting (0.5-4 µg/ml) and immunoprecipitation (20-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~14 kDa histone H2B protein. It does not cross-react with other histones.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Human, mouse, rat
Clonality of antibody:
Synthetic peptide corresponding to the N-terminus of human Histone H2B
How to handle this antibody:
The antibody solution should be gently mixed before use.
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.