-
Available ordering format
Liquid
-
Immunogen
synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
-
Raised in
Mouse
-
Clonality
Monoclonal
-
Clone
IgG2B (clone 2E7)
-
Purification
Affinity purified
-
How to reconstitute
See included datasheet or contact our support service
-
Storage condition
store at -20°C for 1 year; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
western blot (WB), immunofluorescence (IF), immunogold (IG)
-
Connected products
AS10 683 | HDEL | endoplasmic reticulum retention signal mouse antibody, 100 µgAS13 2665 | KDEL | endoplasmic reticulum retention signal (50 µg), rabbit antibodycollection of antibodies for endomembrane proteinsPlant and algal protein extraction bufferSecondary antibodies
-
Recommended dilutions for use
1: 100 - 1. 1000 (WB), 1. 50- 1: 500 (IF)
-
Molecular weight expected аpparent
78 kDa
-
Verified reactivity
Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata, S. cerevisiae, D. melanogaster
-
Possible reactivity
not analyzed
-
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
-
Supplementary information
antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992
-
References
Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697. Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
-
Scientific context
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
-
Notes
protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
-
Protein number
Refer to NCBI
-
TAIR number
Refer to NCBI
-
