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Synonyms
EphB4, HTK, MYK1, TYRO11, Ephrin-type-B-receptor-4.
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Alternative_names
EphB4, HTK, MYK1, TYRO11, Ephrin-type-B-receptor-4.
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Description
A protein playing an essential role in vascular development.
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥97%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rhEPHB4 at 2 ug/ml (100 ul/well) can bind human EphrinB2 with a linear range of 0.8 - 85 ng/ml.
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Results
Measured by its binding ability in a functional ELISA. Immobilized rhEPHB4 at 2 ug/ml (100 ul/well) can bind human EphrinB2 with a linear range of 0.8 - 85 ng/ml.
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Molecular Weight
This protein contains C-terminal polyhistidine tag and has a calculated MW of 57.8 kDa. As a result of glycosylation, DTT-reduced protein migrates as 65 - 70 kDa polypeptide in SDS-PAGE.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Ephrin type-B receptor 4 (EPHB4) is also known as HTK, MYK1 and TYRO11,is a member of Eph family. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EPHB4 binds to Ephrin-B2 and plays an essential role in vascular development. EPHB4 and its ligand ephrin-B2 are specifically expressed on venous and arterial endothelial cells, respectively, and play an essential role in vascular development via bidirectional signals. The forward EPHB4 signaling inhibits cell adhesion, chemotaxis, angiogenesis and tumor growth. In contrast, the reverse Ephrin-B2 signaling exerts the opposite effect. It has been reported that aberrant expression of EPHB4 is associated with prostate cancer and highly malignant breast cancers, accordingly, EPHB4 has potential application as a therapeutic candidate.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
