Amplite Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence*
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Catalog number
11553
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Price
Please ask
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Size
500 Tests
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Description
Amplite Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence* is a kit that is used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors, etc. It provides an optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.
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Shipping
Blue Ice
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Storage
Freezer
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Shelflife
6 months
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Chemical Components
Component A: Amplite™ IR Peroxidase Substrate ; Component B: H2O2 ; Component C: Assay Buffer ; Component D: Horseradish Peroxidase ; Component E: DMSO
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Excitation Wavelength
647 nm
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Emission Wavelength
670 nm
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Personal precautions
Use appropriate protective equipment and methods to clean up spilled substances promptly.
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Uses
Laboratory chemicals *For Research Use Only*
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Ordering
To order Amplite Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence*, please use the Cat. Nr.11553 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence*
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Test
aat supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
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Gene target
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Short name
Amplite Fluorimetric ( ) Assay Kit *Near Infrared *
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Technique
Assay, assays
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Host
Assay
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Label
peroxidase
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Alternative name
Amplite fluorimetry Peroxidase (horseradish peroxidase) test reagent *Near Infrared Fluorescence*
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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