Glycerol Cell-Based Assay Kit

• Catalog number
  K977-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay. Cell-based assay to detect total glycerol in cultured cells. Includes Chloroquine
• Summary
  • Detection method- Fluorescence (Ex/Em 535/587 nm) • Applications-The kit can detect 100 pmol of glycerol in mammalian cell cultures.
• Detection Method
  Fluorescence (Ex/Em 535/587 nm)
• Applications
  The kit can detect 100 pmol of glycerol in mammalian cell cultures.
• Sample Type
  Cell culture supernatants
• Features Benefits
  • Simple procedure; takes ~ 2 hours • Fast and convenient • The kit provides an accurate assay for measuring free glycerol in cell-based assays
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Metabolic diseases such as type II diabetes and fatty liver are becoming more prevalent among an aging population, and are a burden to the healthcare system. Such diseases revolve around the body’s ability to control triglyceride (lipid) metabolism. Events such as fasting can lead to cell autophagy, corresponding with removal of lipid droplets. A number of pathologies can lead to steatosis, bringing about triacylglyceride buildup and release of free fatty acids and free glycerol. Glycerol molecules can then be reabsorbed by the liver or kidney and can be metabolized to re-enter the energy store by pathways such as glycolysis and gluconeogenesis. The glycerol concentration can thus be used as a measure of lipid metabolism and fat mobilization. BioVision’s Glycerol Cell-Based Assay Kit provides a simple, sensitive, straight-forward assay for determination of glycerol concentrations in various cell and tissue culture samples. The assay uses a glycerol-specific enzymatic reaction to convert the probe into a fluorescent product in the presence of glycerol. Glycerol levels can be detected as low as 50 pmol per well, or 1.0 µM in culture medium. Chloroquine (included in the kit as a control) induces steatosis and blocks autophagy, thus leading to a buildup of lipid droplets and an increase in the free glycerol concentration.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Glycerol   Based   Kit  
• Short name
  Glycerol -Based Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Glycerol cellular-Based test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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