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Availability
Please contact us to check the availability
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Immunogen
Purified collagen type I from murine skin
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Specificity
Mouse collagen type I 100%, mouse collagen type II and IV
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Species reactivity
Mouse; human, chicken and rat collagen type I < 0.1%
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Product presentation
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
IgG
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Antibody s clone
polyclonal
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Product is supplied as
purified, lyophilized
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Recommended concentration for use
IFA ≥ 1:80, IHC(P) ≥ 1:500, ELISA ≥ 1:200 (OD ≥ 500)
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Protein concentration
app. 1 mg/ml
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Positive control
Mouse skin
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Pretreatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
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Tested applications
IHC(P), IFA, ELISA, RIA, IB/WB
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Recommended secondary reagent
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7401).
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Storage method
Store at -20°C
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UniProt number
P11Go to UniProt/SwissProt87
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Litterature
1. Demarchez M., Hartmann D.J., Herbage D., Ville G., Prunieras M. (1987) Wound-healing of human-skin transplanted onto the nude-mouse. 2. an immunohistological and ultrastructural study of the epidermal basement-membrane zone reconstruction and connective-t 2. Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A. (1987) The interstitial matrix of human carcinomas and sarcomas transplanted to the nude mouse : immunolocalization of some human and murine components. Cell. Mol. Biol. 33, 647-6 3. Andujar M.B., Hartmann D.J., Emonard H., Magloire H. (1988) Distribution and synthesis of type I and type III collagens in developing mouse molar tooth root. Histochemistry 88, 131-140. 4. Plenat F., Vignaud J.M., Guerret-Stocker S., Hartmann D., Duprez K., Duprez A. (1992) Host-Donor interactions in healing of human split-thickness skin grafts onto nude mice: in situ hybridization, immunohistochemical, and histochemical studies. Transplant
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Gene
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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Group
Polyclonals and antibodies
