-
Availability
Please contact us to check the availability
-
Immunogen
Purified collagen type I from human skin
-
Specificity
Human collagen type I, cross-reactivity with collagen type II and IV
-
Species reactivity
Human, cross-reactivity with cattle
-
Product presentation
affinity purified antibody in phosphate buffered solution containing sodium azide (0.09%) as preservative
-
Product category
Primary Antibody
-
Antibody s raised in
Rabbit
-
Antibody s isotype
IgG
-
Antibody s clone
polyclonal
-
Product is supplied as
liquid concentrate
-
Recommended concentration for use
IFA 1:80, IHC(P) 1:200 - 1:600, ELISA >1:200 (OD>500)
-
Protein concentration
app. 1 mg/ml
-
Positive control
Human skin
-
Pretreatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
-
Tested applications
IHC(P), IFA, ELISA, RIA, IB/WB
-
Recommended secondary reagent
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7401).
-
Storage method
Store at -20°C
-
UniProt number
PGo to UniProt/SwissProt2452 (CO1A1_HUMAN); PGo to UniProt/SwissProt8123 (CO1A2_HUMAN)
-
Litterature
1. Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N. (1980) Collagen immunotyping in human liver: Light and electron microscope study. J. Histochem. Cytochem. 28, 1145-1156. 2. Magloire H., Joffre A., Grimaud J.A., Herbage D., Couble M.L., Chavrier C. (1982) Distribution of type III collagen in the pulp parenchyma of the human developing tooth. Light and electron microscope immunotyping. Histochemistry 74, 319-328. 3. Savino W., Itoh T., Imhof B.A., Dardenne M. (1986) Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines. Thymus 8, 245-256. 4. Bedossa P., Bacci J., Lemaigre G., Martin E. (1987) Effects of fixation and processing on the immunohistochemical visualisation of type-I, -III and -IV collagen in paraffin-embedded liver tissue. Histochemistry 88, 85-89.
-
Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Gene
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Group
Polyclonals and antibodies
-
Latin name
Oryctolagus cuniculus
