Recombinant Mouse SELP / selectin P / P-selectin Protein (His tag)
-
Catalog number
PKSM040507-50µg
-
Price
Please ask
-
Size
50µg
-
-
Synonym
CD62P;GMP-140;Grmp;LECAM3;PADGEM
-
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 cells.When 5 x 10E4 cells/well are added to SELP-coated plates (5 μg/mL and 100 μL/well), approximately >70% cells will adhere specifically after 60 minutes at 37℃.
-
Sequence
Met1-Ala709
-
Fusion tag
C-His
-
Accession
Q01102
-
Expressed Host
HEK293 Cells
-
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
-
Purity
> 95 % as determined by SDS-PAGE
-
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
74 kDa
-
AP Mol Mass
116 kDa
-
Formulation
Lyophilized from sterile PBS, pH 7.4
-
Background
P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.
-
-
Conjugation
histidine
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
SELENOP, SELP
-
Short name
Recombinant Mouse SELP / selectin P / P-selectin Protein (His tag)
-
Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
Host
mouse
-
Label
His
-
Species
Mouse, Mouses
-
Alternative name
Rec. Mouse selectin P (granule membrane protein 140kDa, antigen CD62) / selectin P / P-selectin Protein (histidine detection labelled)
-
Alternative technique
rec, murine
-
Alternative to gene target
selectin P (granule membrane protein 140kDa, antigen CD62), CD62 and CD62P and GMP140 and GRMP and LECAM3 and PADGEM and PSEL, SELP and IDBG-104675 and ENSG00000174175 and 6403, oli this GO saccharide binding, nuclei, Selp and IDBG-202346 and ENSMUSG00000026580 and 20344, SELP and IDBG-631778 and ENSBTAG00000020755 and 281486
-
Gene info
-
Identity
-
Gene
-
Long gene name
selenoprotein P
-
Synonyms gene
-
Synonyms gene name
- selenoprotein P, plasma, 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1996-10-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
selectin P
-
Synonyms gene
-
Synonyms gene name
- selectin P (granule membrane protein 140kD, antigen CD62)
- selectin P (granule membrane protein 140kDa, antigen CD62)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1989-06-30
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- C-type lectin domain containing
- CD molecules
- Sushi domain containing
- Selectins
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products