Rabbit IgG Isotype Control Polyclonal Antibody PE Conjugate

• Catalog number
  N1001PE-25UG
• Price
  Please ask
• Size
  0.025mg

• Category
  Antibody
• Concentration
  0.5 mg/ml in 1X PBS with 0.05% sodium azide
• Form
  Purified
• Conjugation
  PE Conjugate
• Clone
  Polyclonal antibody
• Recognised antigen
  Rabbit IgG Isotype Control PE Conjugate
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  isotype control Igs
• Tested applications
  FACS, IHC, IF
• Recommended dilutions
  FACS: assay dependent,IHC: assay dependent,IF: assay dependent
• Notes
  Generally to be used at the same concentration as the sample being controlled for.
• Added buffer
  1X Phosphate Buffered Saline (PBS), pH 7.4
• Intented use
  This Rabbit IgG isotype control antibodyis to be used only for research purposes and not for diagnostics..
• Gene ID
  NA
• Purity
  Purified by protein A Affinity Chromatography
• Description
  Isotype control antibodies are negative controls used to measure non-specific binding or background levels of a primary antibody in different cell types. They are not specific to any target in a cell, yet retain all non-specific characteristics of the antibodies they control.
• Immunogen
  This is purified native rabbit sera and as such there was no immunogen.
• Storage
  Store the Rabbit IgG isotype control antibody at 4oC. Do not freeze; protect from light.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Additional description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by NJS poly. Rabbit antibodies are very stable and can be stored for several days at room temperature. NJS poly adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Group
  isotypes, Polyclonals and antibodies
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  Conjugate  
• Short name
  IgG Isotype Control
• Technique
  Polyclonal, Antibody, Control, Isotype, Rabbit, IgG, antibodies against human proteins, antibodies for, controls, IgGs, Isotypes and controls, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Rabbit IgG, IgG
• Label
  PE
• Alternative name
  Rabbit IgG Isotype Control PE Conjugate
• Alternative technique
  polyclonals, antibodies, control, controls, rabbit-anti, immunoglobulins
• Tissue
  control

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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