Human CellExp™ FLT-3 Ligand, Mouse Recombinant

  • Catalog number
    P1382-50
  • Price
    Please ask
  • Size
    50 µg
  • Synonyms
    FLT3LG, FL, FLT3L, Flt3 ligand
  • Alternative_names
    FLT3LG, FL, FLT3L, Flt3 ligand
  • Description
    A member of a small family of growth factors that stimulate the proliferation of hematopoietic cells
  • Source
    HEK 293 cells
  • Purity by SDS PAGE
    >90% as determined by SDS-PAGE.
  • Endotoxin Level
    Less than 1.0 EU per ug by the LAL method
  • Activity Specifications test method
    Immobilized Mouse Flt-3 Ligand, His Tag at 1 ug/mL (100 uL/well) can bind Human Flt-3/Flk-2 Fc Chimera with a linear range of 1-5 ng/mL
  • Molecular Weight
    The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    Lyophilized
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. It is recommended to store at -20°C.
  • Background Information
    FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.
  • Amino acid sequence
    AA Gly 27 - Arg 188
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional description
    FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    FLT1, FLT3, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12, MIR9-3
  • Short name
    CellExp™ FLT-3 Ligand, Mouse Recombinant
  • Technique
    Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    mouse
  • Species
    Humans, Mouses
  • Alternative name
    H. sapiens CellExp™ FLT-3 Ligand, Mouse Rec.
  • Alternative technique
    rec, murine
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    fms related receptor tyrosine kinase 3
  • Synonyms gene name
    • fms-related tyrosine kinase 3
    • fms related tyrosine kinase 3
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1990-07-30
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Immunoglobulin like domain containing
    • Receptor tyrosine kinases
    • CD molecules
  • VEGA ID
  • Locus Specific Databases
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 14
  • Locus
    3
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 12
  • Locus
    3
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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