BRD1 bromodomain (556-688 aa) (His-Tag), human recombinant

• Catalog number
  7405-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Bromodomain containing 1, DKFZp686F0325, BR140-like protein, BRL, BRPF1, BRPF2, Bromodomain & PHD finger-containing protein 2
• Alternative_names
  Bromodomain containing 1, DKFZp686F0325, BR140-like protein, BRL, BRPF1, BRPF2, Bromodomain & PHD finger-containing protein 2
• Description
  Protein controlling chromatin structure and gene expression
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Molecular Weight
  17.8 kDa (156 aa, 556-688 aa + His Tag), confirmed by MALDI-TOF.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml in Phosphate buffer saline (pH 7.4) containing 10% glycerol and 1mM DTT.
• Background Information
  The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRD1 is a bromodomain containing protein that has been identified as a susceptibility gene in neurological disorders, such as schizophrenia and bipolar affective disorder. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling. Recombinant human BRD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSEQVAMEL RLTPLTVLLR SVLDQLQDKD PARIFAQPVS LKEVPDYLDH IKHPMDFATM RKRLEAQGYK NLHEFEEDFD LIIDNCMKYN ARDTVFYRAA VRLRDQGGVV LRQARREVDS IGLEEASGMH LPERPA
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  BRD1   bromodomain   556-688   His-Tag  
• Gene symbol
  BRD1, MIR556
• Short name
  BRD1 bromodomain (556-688 aa) (His-Tag), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  BRD1 bromodomain (556-688 aa) (histidine-detection labelled), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1102
• Gene
  BRD1
• Long gene name
  bromodomain containing 1
• Synonyms gene name
  • bromodomain-containing 1
• Synonyms
  • BRL
  • BRPF2
• Synonyms name
  • BR140-like
• GenBank acession
  • AF005067
• Locus
  22q13.33
• Discovery year
  1999-10-29
• Entrez gene record
  23774
• Pubmed identfication
  • 10591208
  • 10602503
• RefSeq identity
  • NM_014577
• Classification
  • PWWP domain containing
  • Bromodomain containing
  • PHD finger proteins
• VEGA ID
  OTTHUMG00000150288

Gene info

• Identity
  HGNC:32812
• Gene
  MIR556
• Long gene name
  microRNA 556
• Synonyms gene
  • MIRN556
• Synonyms
  • hsa-mir-556
• Locus
  1q23.3
• Discovery year
  2006-05-16
• Entrez gene record
  693141
• RefSeq identity
  • NR_030283
• Classification
  • MicroRNAs

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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