EGF

Shipping_Temperature

Ambient
Product_State

Lyophilized
Formulation_Description

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, pH 7.0.
Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin

Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Background

Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.
Alternative Names

Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
Expression system

E.coli

Gene target

EGF
Gene symbol

EGF
Short name

EGF
Species

Human
Alternative name

epidermal growth factor
Alternative to gene target

epidermal growth factor, HOMG4 and URG, EGF and IDBG-34100 and ENSG00000138798 and 1950, transmembrane receptor protein tyrosine kinase activator activity, Extracellular, Egf and IDBG-193432 and ENSMUSG00000028017 and 13645