Bromelain, Bromelain, Affinity purified

• Catalog number
  AS09 554
• Price
  Please ask
• Size
  1 mg

• Available ordering format
  Lyophilized in phosphate buffer saline
• Immunogen
  native bromelain isolated and purified from Ananas comosus
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified IgG
• How to reconstitute
  For reconstitution please add 0.5 ml of sterile destilled water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
• Connected products
  AS09 552 | anti-bromelainAS09 553 | anti-bromelain (biotinylated)AS09 546 | anti-PPI | papain (biotinylated)AS09 547 | anti-PPI | papain (affinity purified)Plant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 1000 - 1: 100 000 for techniques listed above
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  Ananas comosus
• Possible reactivity
  Ananas comosus
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
• References
  to be added when available
• Scientific context
  Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
• Notes
  antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Test
  A high affinity purification column was use to purify Bromelain, Bromelain, by agisera by chromatographic size exclusion.
• Description
  1 of protein A or G purified by agisera will give more specificity than crude serum.

• Gene target
  Bromelain   Bromelain  
• Short name
  Bromelain, Bromelain,
• Technique
  affinity, purified, purifications
• Alternative name
  Bromelain, Bromelain, protein enriched
• Alternative technique
  pure

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