Anti-p150 CAF1 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-9392R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  p150 CAF1
• Specificity
  This antibody reacts specifically with p150 CAF1
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human p150 CAF1
• Gene ID
  10036
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  CAF-1 p150; CAF 1 150 kDa subunit; CAF 1; CAF 1 subunit A; CAF; CAF I 150 kDa subunit; CAF I p150; CAF Ip150; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; CAF1 150 kDa subunit; CAF1; CAF1 p150 Subunit; CAF1 subunit A; CAF1A; CAF1A; CAF1A_HUMAN; CAF1B; CAF1P150; CAF1P155; CAF1P155; CHAF1A; Chromatin assembly factor 1 subunit A; Chromatin Assembly Factor 1 Subunit A p150; Chromatin assembly factor I 150 kDa; Chromatin assembly factor I 150 kDa; Chromatin Assembly Factor I; Chromatin assembly factor I p150 subunit; DCAF1; hp150; Nucleosome Remodeling Factor 150kDa Subunit; NURF150; P150.
• Also known as
  p150 CAF1 Polyclonal Antibody
• Other name
  Anti-p150 CAF1 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  p150   CAF1  
• Gene symbol
  CNOT7, CNOT8, CHAF1A, CHAF1B
• Short name
  Anti-p150 CAF1
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  p150 CAF1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:14101
• Gene
  CNOT7
• Long gene name
  CCR4-NOT transcription complex subunit 7
• Synonyms gene
  • CAF1
• Synonyms gene name
  • CCR4-NOT transcription complex, subunit 7
• Synonyms name
  • BTG1 binding factor 1
• GenBank acession
  • L46722
• Locus
  8p22
• Discovery year
  2000-12-01
• Entrez gene record
  29883
• Pubmed identfication
  • 10637334
  • 1538749
  • 17264152
• RefSeq identity
  • NM_013354
• Classification
  • CCR4-NOT transcription complex
• VEGA ID
  OTTHUMG00000096971

Gene info

• Identity
  HGNC:9207
• Gene
  CNOT8
• Long gene name
  CCR4-NOT transcription complex subunit 8
• Synonyms gene
  • POP2
• Synonyms gene name
  • CCR4-NOT transcription complex, subunit 8
• Synonyms
  • CAF1
  • hCAF1
  • CALIF
• Synonyms name
  • PGK promoter directed over production
• GenBank acession
  • AF053318
• Locus
  5q33.2
• Discovery year
  1998-09-25
• Entrez gene record
  9337
• Pubmed identfication
  • 10036195
  • 10637334
• RefSeq identity
  • NM_004779
• Classification
  • CCR4-NOT transcription complex
• VEGA ID
  OTTHUMG00000130192

Gene info

• Identity
  HGNC:1910
• Gene
  CHAF1A
• Long gene name
  chromatin assembly factor 1 subunit A
• Synonyms gene name
  • chromatin assembly factor 1, subunit A (p150)
• Synonyms
  • CAF1P150
  • CAF1B
  • CAF-1
  • CAF1
  • P150
  • MGC71229
• Synonyms name
  • chromatin assembly factor I (150 kDa)
• GenBank acession
  • U20979
• Locus
  19p13.3
• Discovery year
  1999-09-29
• Entrez gene record
  10036
• Pubmed identfication
  • 7600578
• RefSeq identity
  • NM_005483
• VEGA ID
  OTTHUMG00000181922

Gene info

• Identity
  HGNC:1911
• Gene
  CHAF1B
• Long gene name
  chromatin assembly factor 1 subunit B
• Synonyms gene name
  • chromatin assembly factor 1, subunit B (p60)
• Synonyms
  • CAF1P60
  • CAF-1
  • CAF1
  • CAF1A
  • MPP7
  • MPHOSPH7
• Synonyms name
  • M-phase phosphoprotein 7
  • Chromatin assembly factor I, p60 subunit
  • human chromatin assembly factor-I p60 subunit
• GenBank acession
  • U20980
• Locus
  21q22.12-q22.13
• Discovery year
  1999-09-29
• Entrez gene record
  8208
• Pubmed identfication
  • 7600578
  • 8792829
• RefSeq identity
  • NM_005441
• Classification
  • WD repeat domain containing
• VEGA ID
  OTTHUMG00000086606

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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