MSK1 (phospho-Thr581) polyclonal antibody

• Catalog number
  AP0095-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; MSK1
• Immunogen
  Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Threonine 581.
• Swiss Prot
  O75582
• Reactivity
  Human,Mouse,Rat
• Applications
  WB
• Application_all
  WB: 1:500~1:2000
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  p-MSK1(Thr581) polyclonal antibody detects endogenous levels of MSK1 protein when phosphorylated at Thr581.
• Usage
  Western blot (WB) analysis of p-MSK1(Thr581) polyclonal antibody at 1:500 or 1:2000 dilution Lane1:H9C2 treated with LPS(40ng/ml) for 45min whole cell lysate Lane2:H9C2 treated with LPS(40ng/ml) for 15min whole cell lysate Lane3:H9C2 treated with LPS(40ng/ml) for 5min whole cell lysate Lane4:L02 treated with LPS(40ng/ml) for 45min whole cell lysate Lane5:L02 treated with LPS(40ng/ml) for 15min whole cell lysate Lane6:L02 whole cell lysate Lane7:The Brain tissue lysate of Mouse(40ug)
• BiowMW
  ~ 90 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  MSK1   phospho-Thr581  
• Gene symbol
  RPS6KA5
• Short name
  MSK1 (phospho-Thr581) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  MSK1 (phosphorilated-Thr581) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:10434
• Gene
  RPS6KA5
• Long gene name
  ribosomal protein S6 kinase A5
• Synonyms gene name
  • ribosomal protein S6 kinase, 90kD, polypeptide 5
• Synonyms
  • MSK1
  • RLPK
• GenBank acession
  • AF074393
• Locus
  14q32.11
• Discovery year
  1999-03-10
• Entrez gene record
  9252
• Pubmed identfication
  • 9687510
  • 10702687
• RefSeq identity
  • NM_004755
• Classification
  • MAPK activated protein kinases
  • AGC family kinases
• VEGA ID
  OTTHUMG00000171055

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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