GSK-3β, human recombinant
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Catalog number
7004-10
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Price
Please ask
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Size
10 ug
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Synonyms
GSK, Glycogen synthase kinase-3 (GSK-3)
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Alternative_names
GSK, Glycogen synthase kinase-3 (GSK-3)
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Description
A phosphorylating and an inactivating agent of glycogen synthase
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Recombinant
Yes
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Source
E. coli.
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Activity Specifications test method
Activity of GSK-3β was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 µg of the recombinant GSK-3β in a 20 µl reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerol phosphate, 12 mM MgCl₂, 2 mM DTT, 0.1 M Na3VO4, and 200 µM ATP. After 30 minutes incubation at 30°C, phosphorylation of Tau by the recombinant GSK-3β was determined by Western blot using a phospho-Tau antibody.
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Molecular Weight
53.0-56.0 kDa
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Storage Temp
-70°C
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Shipping
dry ice
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized from 2 mg/ml in 0.1 M NH4CO3
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Reconstitution Instructions
Reconstitute to 1 mg/ml in PBS containing 15% glycerol. The solution should be stored at –70°C, stable for up to 3 months.
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Background Information
Glycogen synthase kinase-3 (GSK-3) is a serine/theronine kinase that was first characterized by its role in intermediary metabolism. In mammals, serine kinase is encoded by two related genes called GSK-3α (51 kDa) and GSK-3β (46 kDa). GSK-3 has been implicated in the regulation of the c-JuP-1 transcription factor and other intracellular signaling pathways.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12, MIR9-3, MIR941-3, PIRC15
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Short name
GSK-3β, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
GSK-3β, H. sapiens Rec.
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
microRNA 1302-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 509-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 7-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 9-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 941-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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