Thrombin Cleavage Kit

• Catalog number
  K377-500
• Price
  Please ask
• Size
  500 assays

• Description
  Robust and efficient cleavage of tags from target proteins containing the Thrombin recognition sequence.
• Summary
  • SDS-PAGE
• Detection Method
  SDS-PAGE
• Applications
  • Efficiently removes tags from recombinant fusion proteins containing accessible Thrombin-specific recognition sequence.
• Sample Type
  Fusion or affinity-tagged protein containing Thrombin-specific cleavage site.
• Features Benefits
  • Simple procedure, fast and efficient cleavage of tags • The kit contains an active Thrombin enzyme sufficient to cleave up to 5 mg of target protein. • A 6x His-tagged protein containing the Thrombin cleavage site is included as a control. • Following cleavage of the target protein, Thrombin can be conveniently removed using a Heparin Sepharose column.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Thrombin enzyme (Factor IIa, EC 3.4.21.5) is an important clotting factor that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. It is also used as a valuable biochemical tool due to its high proteolytic specificity. Thrombin cleavage site (e.g. Leu-Val-Pro-Arg-ll-Gly-Ser; ll: cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. After successful cleavage with Thrombin, affinity tags or fused proteins can be separated from the target protein. Biovision's Thrombin Cleavage Kit provides an easy approach to test and optimize cleavage conditions of a target fusion or affinity-tagged protein containing Thrombin-specific cleavage site. The kit contains an active Thrombin enzyme sufficient to cleave up to 5 mg of the target protein. A 6xHis-tagged protein containing the Thrombin cleavage site is included as a Cleavage Control Protein. Following cleavage of the target protein, Thrombin can be removed by passing the reaction mix through the Heparin Sepharose column (Catalog # 6553 and 6554).
• Data Sheet Link

• Gene target
  Thrombin   Cleavage   Kit  
• Short name
  Thrombin Cleavage Kit
• Alternative name
  Thrombin Cleavage reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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