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Target antigen
Mitochondrial Pyruvate dehydrogenase kinase 1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1(408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Mitochondrial Pyruvate dehydrogenase kinase 1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Mitochondrial Pyruvate dehydrogenase kinase 1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
PDK1(Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.
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Related articles
1. Gudi, R., Bowker-Kinley, M. M., Kedishvili, N. Y., Zhao, Y., Popov, K. M. Diversity of the pyruvate dehydrogenase kinase gene family in humans. J. Biol. Chem. 270: 28989-28994, 1995.
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Gene Name
PDK1
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Protein Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
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Gene Full Name
pyruvate dehydrogenase kinase, isozyme 1
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Synonyms
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 antibody|[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody|HGNC:8809 antibody|mitochondrial antibody|Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody|PDH kinase 1 antibody|Pdk1 antibody|PDK1_HUMAN antibody|Pyruvate dehydrogenase kinase isoform 1 antibody|Pyruvate dehydrogenase kinase, isoenzyme 1 antibody
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Uniprot ID
Q15118
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps