If you buy Antibodies supplied by EpiGentek they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
UBE2K Polyclonal Antibody, Conjugated
UBE2K polyclonal (Antibody to), biotinilated coupled
Long gene name ubiquitin conjugating enzyme E2 K
Synonyms gene name
- huntingtin interacting protein 2
- ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
- ubiquitin-conjugating enzyme E2K
Discovery year 1997-08-28
Entrez gene record 3093
- Ubiquitin conjugating enzymes E2
Havana BLAST/BLAT OTTHUMG00000160543