-
Synonyms
Apolipoprotein E3
-
Alternative_names
Apolipoprotein E3, APOE, apolipoprotein, apolipoproteins
-
Description
Apolipoprotein essential for the normal catabolism of triglyceride-rich lipoprotein constituents
-
Recombinant
Yes
-
Source
E. coli
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Purity
≥90%
-
Biological activity
Test in process
-
Molecular Weight
34 kDa
-
Storage Temp
-20°C
-
Shipping
Blue ice
-
Shelf Life
12 months
-
Appearance
Lyophilized protein
-
Physical form description
Sterile filtered and lyophilized from 20 mM Sodium Phosphate
-
Reconstitution Instructions
Reconstitute in dH₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.
-
Background Information
ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population. Recombinant human ApoE3 is a 34.0 kDa protein containing 299 amino acid residues. This protein has a N-terminal His-tag.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants