OCT4 (OCT3) Antibody

• Catalog number
  6765-100
• Price
  Please ask
• Size
  100 μl

• Antibody Target
  Oct4/Oct3
• Target Alternative Name
  POU5F1; OCT3; OCT4; OTF3; POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3
• Antibody Type
  Polyclonal
• Species Reactivities
  Human.
• Immunogen Sequence
  This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-141 amino acids from human OCT4 (OCT3).
• Accession
  Q01860
• Gene Id
  5460
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification
  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Application
  Western blot, IF.
• Positive Control
  Western blot: HL-60 cell lysate, IF: Sy5Y cells
• Application And Usages
  Western blot: ~1:1000, IF: ~1:10–1:50.
• Description
  Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation. Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker. Oct-4 exists as two splice variants, Oct-4A and Oct-4B. Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  OCT4   OCT3  
• Gene symbol
  POU5F1, POU5F1P5, SLC22A3, POU5F1P4
• Short name
  OCT4 (OCT3) Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  OCT4 (OCT3) (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9221
• Gene
  POU5F1
• Long gene name
  POU class 5 homeobox 1
• Synonyms gene
  • OTF3
• Synonyms gene name
  • POU domain class 5, transcription factor 1
• Synonyms
  • OCT3
  • Oct4
  • MGC22487
• GenBank acession
  • Z11898
• Locus
  6p21.33
• Discovery year
  1992-11-05
• Entrez gene record
  5460
• Pubmed identfication
  • 1408763
• RefSeq identity
  • NM_002701
• Classification
  • POU class homeoboxes and pseudogenes
• VEGA ID
  OTTHUMG00000031206

Gene info

• Identity
  HGNC:33311
• Gene
  POU5F1P5
• Long gene name
  POU class 5 homeobox 1 pseudogene 5
• Synonyms gene name
  • POU domain class 5, transcription factor 1 pseudogene 5
• Synonyms
  • Oct4-pg5
• Locus
  10q21.3
• Discovery year
  2007-02-07
• Entrez gene record
  100009667
• Classification
  • POU class homeoboxes and pseudogenes
• VEGA ID
  OTTHUMG00000018342

Gene info

• Identity
  HGNC:10967
• Gene
  SLC22A3
• Long gene name
  solute carrier family 22 member 3
• Synonyms gene name
  • solute carrier family 22 (extraneuronal monoamine transporter), member 3
  • solute carrier family 22 (organic cation transporter), member 3
• Synonyms
  • OCT3
  • EMT
• Synonyms name
  • organic cation transporter 3
  • extraneuronal monoamine transporter
• GenBank acession
  • AF078749
• Locus
  6q25.3
• Discovery year
  1998-07-16
• Entrez gene record
  6581
• Pubmed identfication
  • 9632645
  • 9933568
• RefSeq identity
  • NM_021977
• Classification
  • Solute carriers
• VEGA ID
  OTTHUMG00000015953

Gene info

• Identity
  HGNC:33310
• Gene
  POU5F1P4
• Long gene name
  POU class 5 homeobox 1 pseudogene 4
• Synonyms gene name
  • POU domain class 5, transcription factor 1 pseudogene 4
• Synonyms
  • POU5FLC1
  • Oct4-pg4
• Locus
  1q22
• Discovery year
  2007-02-07
• Entrez gene record
  645682
• Classification
  • POU class homeoboxes and pseudogenes
• VEGA ID
  OTTHUMG00000014016

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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