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Human Glycosylphosphatidylinositol ELISA Kit 96 well plate

Human Glycosylphosphatidylinositol ELISA Kit 96 well plate is available 9 times from Bioma labs

EKU04535 | Human Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) ELISA Kit, 96 well plate size: 10x96T | 8,366.20 USD

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EKU04535 | Human Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) ELISA Kit, 96 well plate size: 5x96T | 4,627.16 USD

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EKU04535 | Human Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) ELISA Kit, 96 well plate size: 96T | 1,098.33 USD

Catalog number EKU04535
Supplier bioma
Price 1,098.33 USD
Size 96T
Detection limits 0.625-10 ng/mL
Sensitivity limit 0,242 ng/mL
Samples to be analyzed Tissue homogenates, cell lysates and other biological fluids
Protein number Please refer to Uniprot
Gene number Please refer to GenBank
Gene name Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1
Alternate gene name GPI-HBP1; High density lipoprotein-binding protein 1
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1). No significant cross-reactivity or interference between Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) and analogues was observed.
Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Research main area Metabolic pathway;
Description High and low density proteins are supplied by bioma in volumes of 1. Other densities are possible.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene targetGlycosylphosphatidylinositol Anchored Lipoprotein Binding Protein GPIHBP1
Short name Glycosylphosphatidylinositol Anchored Lipoprotein Binding Protein 1 (GPIHBP1) ELISA Kit, 96
Alternative name H. sapiens Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
1. Gene info
Identity 24945
Gene GPIHBP1
Long gene name glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1
Synonyms gene name
  • GPI anchored high density lipoprotein binding protein 1
Synonyms
  • LOC338328
  • GPI-HBP1
Synonyms name
  • endothelial cell LPL transporter
GenBank acession
  • AF088057
Locus 8q24.3
Discovery year 2008-02-07
Entrez gene record 338328
Pubmed identfication
  • 12496272
  • 17883852
  • 17620854
  • 17403372
RefSeq identity
  • NM_178172
Classification
  • LY6/PLAUR domain containing
Havana BLAST/BLAT OTTHUMG00000164950
Product images
    #Human#.jpg

EKU04536 | Human Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) ELISA Kit, 96 well plate size: 5x96T | 4,212.63 USD

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EKU04536 | Human Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) ELISA Kit, 96 well plate size: 96T | 1,002.67 USD

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EKU04536 | Human Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) ELISA Kit, 96 well plate size: 10x96T | 7,611.55 USD

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EKU04537 | Human Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) ELISA Kit, 96 well plate size: 10x96T | 7,611.55 USD

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EKU04537 | Human Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) ELISA Kit, 96 well plate size: 96T | 1,002.67 USD

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EKU04537 | Human Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) ELISA Kit, 96 well plate size: 5x96T | 4,212.63 USD

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Verified reactivity Homo sapiens (Human)
Assay duration 3 hours
Verified applications ELISA
Assay class Sandwich
ELISA detection Colorimetric
Alternate protein number Please refer to SwissProt
Estimated production time 7-11 business days
Shipping requirements Blue ice
Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Use for 8 months
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Protocol Please see ELISA's datasheet, otherwise contact us
ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes For research use only. Not for diagnostic procedures.
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Human
Alternative technique elisas
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Glycosylphosphatidylinositol Anchored Lipoprotein Binding Pr
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EU:+32-(0)1-658-90-45 US:+1-(408)780-0908 [email protected]
  • Glycosylphosphatidylinositol
  • Anchored
  • Lipoprotein
  • Binding
  • Protein
  • GPIHBP1
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