Caspase-3, human recombinant

• Catalog number
  1083-100
• Price
  Please ask
• Size
  100 units

• Synonyms
  Caspase-3
• Alternative_names
  Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1
• Description
  A cysteine-aspartic acid protease involved in apoptosis
• Recombinant
  Yes
• Source
  E. coli.
• Assay
  SDS-PAGE
• Molecular Weight
  large (17 kD) and small (11 kD) subunits
• Storage Temp
  -70°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Semi-Dry
• Physical form description
  Semi-Dry
• Reconstitution Instructions
  Reconstitute in water
• Background Information
  Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme (Full length gene Accession No. NP_004337) . The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Caspase-3  
• Gene symbol
  CASP1P3, CASP3, CASP3P1
• Short name
  Caspase-3, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  caspase-3, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:43777
• Gene
  CASP1P3
• Long gene name
  caspase 1 pseudogene 3
• Synonyms gene name
  • caspase 1, apoptosis-related cysteine peptidase pseudogene 3
• Locus
  11q22.3
• Discovery year
  2012-02-24
• Entrez gene record
  100422634
• RefSeq identity
  • NG_024409

Gene info

• Identity
  HGNC:1504
• Gene
  CASP3
• Long gene name
  caspase 3
• Synonyms gene name
  • caspase 3, apoptosis-related cysteine protease
  • caspase 3, apoptosis-related cysteine peptidase
• Synonyms
  • CPP32
  • CPP32B
  • Yama
  • apopain
• GenBank acession
  • BC016926
• Locus
  4q35.1
• Discovery year
  1996-07-22
• Entrez gene record
  836
• Pubmed identfication
  • 8780721
• RefSeq identity
  • NM_004346
• Classification
  • Caspases
  • Small nucleolar RNA protein coding host genes
• VEGA ID
  OTTHUMG00000133681

Gene info

• Identity
  HGNC:43596
• Gene
  CASP3P1
• Long gene name
  caspase 3 pseudogene 1
• Synonyms gene name
  • caspase 3, apoptosis-related cysteine peptidase pseudogene 1
• Locus
  1p31.1
• Discovery year
  2011-11-22
• Entrez gene record
  100131616
• RefSeq identity
  • NG_022722
• VEGA ID
  OTTHUMG00000009345

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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