Glucose Uptake Colorimetric Assay Kit

  • Catalog number
    K676-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Non-radioactive, Highly Sensitive Assay
  • Summary
    • Detection method- Absorbance (412 nm) • Species reactivity- Mammalian • Application- This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.
  • Sample Type
    Living cells (suspension and adherent)
  • Features Benefits
    • Simple procedure • Fast and convenient • The assay is easy, non-radioactive, and highly sensitive
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In BioVision’s glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.
  • Test
    Biovision supplies other types of Assays as 1.
  • Properties
    Colorimetric assays or detection use UV absorption or enzymatic color reaction.
  • Gene target
    Glucose   Uptake   Kit  
  • Short name
    Glucose Uptake Colorimetric Assay Kit
  • Technique
    colorimetric, Assay, assays, colorimetry
  • Host
    Assay
  • Alternative name
    Glucose Uptake colorimetry test reagent
  • Alternative technique
    assays, arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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