ZNF521 Antibody (NT)
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Catalog number5406-100
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PricePlease ask
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Size100 µg
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Antibody TargetZNF521
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Target Alternative NameZinc finger protein 521, EHZF, Evi3
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceZNF521 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521.
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AccessionQ96K83
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Gene Id25925
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AppearanceColorless liquid
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FormLiquid
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Concentration1 mg/ml
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Formulation100 µg (1 mg/ml) in 1X PBS containing 1 mg/ml BSA, 50% glycerol and less than 0.02% sodium azide, pH 7.4.
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PurificationAffinity chromatography purified via peptide column
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ApplicationWestern blot, ICC, ELISA
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Positive ControlWestern Blot: HeLa cell Lysate IHC: HeLa cells
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Application And UsagesWestern Blot: 1 μg/ml, ICC: 2.5 μg/ml, ELISA. However, the optimal conditions should be determined individually.
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DescriptionThe zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedVerified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolZNF521
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Short nameZNF521 Antibody (NT)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG1
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Alternative nameZNF521 (antibody to-) (NT)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namezinc finger protein 521
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-04-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data