ZNF521 Antibody (NT)

• Catalog number
  5406-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  ZNF521
• Target Alternative Name
  Zinc finger protein 521, EHZF, Evi3
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  ZNF521 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521.
• Accession
  Q96K83
• Gene Id
  25925
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  100 µg (1 mg/ml) in 1X PBS containing 1 mg/ml BSA, 50% glycerol and less than 0.02% sodium azide, pH 7.4.
• Purification
  Affinity chromatography purified via peptide column
• Application
  Western blot, ICC, ELISA
• Positive Control
  Western Blot: HeLa cell Lysate IHC: HeLa cells
• Application And Usages
  Western Blot: 1 μg/ml, ICC: 2.5 μg/ml, ELISA. However, the optimal conditions should be determined individually.
• Description
  The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  ZNF521  
• Gene symbol
  ZNF521
• Short name
  ZNF521 Antibody (NT)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG1
• Alternative name
  ZNF521 (antibody to-) (NT)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:24605
• Gene
  ZNF521
• Long gene name
  zinc finger protein 521
• Synonyms
  • EHZF
  • Evi3
• Synonyms name
  • early hematopoietic zinc finger
• GenBank acession
  • AK027354
• Locus
  18q11.2
• Discovery year
  2004-04-05
• Entrez gene record
  25925
• Pubmed identfication
  • 11984006
  • 14630787
• RefSeq identity
  • NM_015461
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000179511

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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